Identification of Mcm2 Phosphorylation Sites by S-phase-regulating Kinases
نویسندگان
چکیده
منابع مشابه
Mec1 is one of multiple kinases that prime the Mcm2-7 helicase for phosphorylation by Cdc7.
Activation of the eukaryotic replicative DNA helicase, the Mcm2-7 complex, requires phosphorylation by Cdc7/Dbf4 (Dbf4-dependent kinase or DDK), which, in turn, depends on prior phosphorylation of Mcm2-7 by an unknown kinase (or kinases). We identified DDK phosphorylation sites on Mcm4 and Mcm6 and found that phosphorylation of either subunit suffices for cell proliferation. Importantly, prior ...
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In the mammalian central nervous system, the structure known as the postsynaptic density (PSD) is a dense complex of proteins whose function is to detect and respond to neurotransmitter released from presynaptic axon terminals. Regulation of protein phosphorylation in this molecular machinery is critical to the activity of its components, which include neurotransmitter receptors, kinases/ phosp...
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Injection of cycloheximide into rats induced the incorporation of up to 5 mol of phosphate/mol of liver 40 S ribosomal protein S6. Treatment of the protein with cyanogen bromide generated three phosphopeptides of Mr approximately 31,000, approximately 27,000, and approximately 4,000. Increasing the concentration of cyanogen bromide or redigestion of the larger peptides with additional cyanogen ...
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G protein-coupled receptor kinaseinteracting protein 1 (GIT1) was originally identified as an ADP ribosylation factor GTPase-activating protein (ARF-GAP) that binds Gprotein-coupled receptor kinases (GRKs) and regulates membrane trafficking (Premont et al., 1998). Subsequent studies have shown a much broader function for GIT1 and GIT2/PKL as regulators of migrationrelated processes, including a...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2006
ISSN: 0021-9258
DOI: 10.1074/jbc.m512921200